Identification of Scopoletin and Chlorogenic Acid as Potential Active Components in Sunflower Calathide Enzymatically Hydrolyzed Extract towards Hyperuricemia

It is known that sunflower (Helianthus annuus L.) calathide enzymatically hydrolyzed extract (SCHE) contributes to the regulation of serum uric acid (UA); however, evidence regarding its bioactive components and mechanism are lacking. We identified two water-soluble (scopoletin chlorogenic acid) abundant in calathide, especially evaluated for inhibition xanthine oxidase (XO) expression levels urate transporters with SCHE. Molecular docking a acid–XO complex was more stable than Scopoletin–XO, binding pockets, which closed Mo = S center, similar pockets. Moreover, exhibited stronger scopoletin below 260 μM, despite IC50 (577.7 μM) being lower (844.7 on UA generation assessed by spectrophotometer vitro. revealed were competitive inhibitors XO. In addition, SCHE (300 μg/mL) (0.75 mM) obviously inhibited transporter 1 (URAT1) glucose 9 (GLUT9) levels, while significantly upregulated GLUT9. To summarize, served crucial role consistent functioned as an important ingredient The strategic analysis combined may contribute development food supplemental alternatives metabolism reduction agricultural byproduct waste.